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superoxide dismutase 2 sod2  (Proteintech)


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    Structured Review

    Proteintech superoxide dismutase 2 sod2
    Superoxide Dismutase 2 Sod2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superoxide+dismutase+2+sod2/SOD2+Antibody/pm41828973-75-23-28
    Average 96 stars, based on 499 article reviews
    superoxide dismutase 2 sod2 - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Bu-Shen-Tian-Jing Formula alleviates oxidative-inflammatory stress in granulosa cells of polycystic ovary syndrome through AGEs-RAGE/NOX4/NF-κB pathway.
    Article Snippet: Equal protein aliquots (30 μg/ lane) were resolved electrophoretically on 4–12% Bis– Tris gradient gels (FuturePAGETM, ACE Biotechnology) under denaturing conditions with MOPS-SDS buffer (ACE Biotechnology) using constant voltage (130 V) for 60 min. Proteins were transferred onto methanolactivated 0.22 μm PVDF membranes (Millipore) using a semi-dry transfer system (100 V for 80 min). .. Membranes were blocked with 5% BSA in TBST (20 mM Tris–HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) for 2 h at room temperature, followed by incubation with primary antibodies at 4 °C for 16 h: RAGE (1: 2000, 16,346-1-AP, Proteintech), NOX4 (1: 2000, 14,347-1-AP, Proteintech), superoxide dismutase 2 (SOD2) (1: 5000, 24,127-1-AP, Proteintech), NF-κB p65 (1: 2000, 10,745-1-AP, Proteintech), phospho-NF-κB p65 (Ser468) (1: 2000, 82,335-1- RR, Proteintech), IkBα (1: 1000, PTM-6005, PTM BIO), phospho-IKBα (phospho S32/36) (1: 1000, PTM-6401, PTM BIO), mitogen-activated protein kinase 14 (p38 MAPK) (1: 5000, 14,064-1-AP, Proteintech), phosphop38 MAPK (Thr180/Tyr182) (1: 5000, 28,796-1-AP, Proteintech), β-tubulin (1: 2000, PTM-6414, PTM BIO). .. After TBST washes (3 × 10 min), membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies (1: 5000, Proteintech) for 1 h at 25 °C.

    Article Title: Bu-Shen-Tian-Jing Formula alleviates oxidative-inflammatory stress in granulosa cells of polycystic ovary syndrome through AGEs-RAGE/NOX4/NF-κB pathway
    Article Snippet: Equal protein aliquots (30 μg/lane) were resolved electrophoretically on 4–12% Bis–Tris gradient gels (FuturePAGETM, ACE Biotechnology) under denaturing conditions with MOPS-SDS buffer (ACE Biotechnology) using constant voltage (130 V) for 60 min. Proteins were transferred onto methanol-activated 0.22 μm PVDF membranes (Millipore) using a semi-dry transfer system (100 V for 80 min). .. Membranes were blocked with 5% BSA in TBST (20 mM Tris–HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) for 2 h at room temperature, followed by incubation with primary antibodies at 4 °C for 16 h: RAGE (1: 2000, 16,346-1-AP, Proteintech), NOX4 (1: 2000, 14,347-1-AP, Proteintech), superoxide dismutase 2 (SOD2) (1: 5000, 24,127-1-AP, Proteintech), NF-κB p65 (1: 2000, 10,745-1-AP, Proteintech), phospho-NF-κB p65 (Ser468) (1: 2000, 82,335-1-RR, Proteintech), IkBα (1: 1000, PTM-6005, PTM BIO), phospho-IKBα (phospho S32/36) (1: 1000, PTM-6401, PTM BIO), mitogen-activated protein kinase 14 (p38 MAPK) (1: 5000, 14,064-1-AP, Proteintech), phospho-p38 MAPK (Thr180/Tyr182) (1: 5000, 28,796-1-AP, Proteintech), β-tubulin (1: 2000, PTM-6414, PTM BIO). .. After TBST washes (3 × 10 min), membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies (1: 5000, Proteintech) for 1 h at 25 °C.

    Article Title: Vitamin K2 Alleviates Metabolic Dysfunction-Associated Steatotic Liver Disease Through Mitochondrial Dysfunction Modulation via Gut Microbiota
    Article Snippet: .. The membrane was blocked with non-fat milk and then incubated overnight at 4 °C with primary antibodies against SIRT3 (1:1000, 10099-1-AP, Proteintech, Wuhan, Hubei, China), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) (1:1000, #2178, CST, Beverly, MA, USA), β-Tubulin (1:1000, 80713- 1-RR, Proteintech, Wuhan, China), Catalase (1:1000, 21260-1-AP, Proteintech, Wuhan, Hubei, China), and superoxide dismutase 2 (SOD2) (1:1000, 24127-1-AP, Proteintech, Wuhan, Hubei, China). .. The membranes were washed three times with Tris-buffered saline + Tween 20 (TBST) buffer, followed by a 2-hour incubation with HRP conjugated secondary anti-rabbit (1:1000, #7073, CST) or anti-mouse antibodies (1:1000, #7076, CST).

    Membrane:

    Article Title: Vitamin K2 Alleviates Metabolic Dysfunction-Associated Steatotic Liver Disease Through Mitochondrial Dysfunction Modulation via Gut Microbiota
    Article Snippet: .. The membrane was blocked with non-fat milk and then incubated overnight at 4 °C with primary antibodies against SIRT3 (1:1000, 10099-1-AP, Proteintech, Wuhan, Hubei, China), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) (1:1000, #2178, CST, Beverly, MA, USA), β-Tubulin (1:1000, 80713- 1-RR, Proteintech, Wuhan, China), Catalase (1:1000, 21260-1-AP, Proteintech, Wuhan, Hubei, China), and superoxide dismutase 2 (SOD2) (1:1000, 24127-1-AP, Proteintech, Wuhan, Hubei, China). .. The membranes were washed three times with Tris-buffered saline + Tween 20 (TBST) buffer, followed by a 2-hour incubation with HRP conjugated secondary anti-rabbit (1:1000, #7073, CST) or anti-mouse antibodies (1:1000, #7076, CST).

    Pyrolysis Gas Chromatography:

    Article Title: Vitamin K2 Alleviates Metabolic Dysfunction-Associated Steatotic Liver Disease Through Mitochondrial Dysfunction Modulation via Gut Microbiota
    Article Snippet: .. The membrane was blocked with non-fat milk and then incubated overnight at 4 °C with primary antibodies against SIRT3 (1:1000, 10099-1-AP, Proteintech, Wuhan, Hubei, China), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) (1:1000, #2178, CST, Beverly, MA, USA), β-Tubulin (1:1000, 80713- 1-RR, Proteintech, Wuhan, China), Catalase (1:1000, 21260-1-AP, Proteintech, Wuhan, Hubei, China), and superoxide dismutase 2 (SOD2) (1:1000, 24127-1-AP, Proteintech, Wuhan, Hubei, China). .. The membranes were washed three times with Tris-buffered saline + Tween 20 (TBST) buffer, followed by a 2-hour incubation with HRP conjugated secondary anti-rabbit (1:1000, #7073, CST) or anti-mouse antibodies (1:1000, #7076, CST).



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